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Genes in Cipro resistant F . tularensis LVS isolates containing mutations identified both by Illumina sequencing and SNP microarray. The reference genome F . tularensis NC_007880 was used to determine the reference genome position. The lists of identified amino acid diversities at the given mutation points observed in the corresponding positions in homologous proteins are provided in the “Amino acid change” column.

Journal: PLoS ONE

Article Title: Identification of Genome-Wide Mutations in Ciprofloxacin-Resistant F . tularensis LVS Using Whole Genome Tiling Arrays and Next Generation Sequencing

doi: 10.1371/journal.pone.0163458

Figure Lengend Snippet: Genes in Cipro resistant F . tularensis LVS isolates containing mutations identified both by Illumina sequencing and SNP microarray. The reference genome F . tularensis NC_007880 was used to determine the reference genome position. The lists of identified amino acid diversities at the given mutation points observed in the corresponding positions in homologous proteins are provided in the “Amino acid change” column.

Article Snippet: A combination of high density tiling microarray, Illumina and Sanger sequencing was used to identify and confirm mutations in these isolates.

Techniques: Illumina Sequencing, Microarray, Mutagenesis, Membrane, CRISPR